October 9, 2020

Separating translation events in the cytosol and on the ER

Ribosome profiling

4: Dephosphorylation and polyadenylation

5: Reverse transcription with TSO

5: Inherent drawback: concatenation

6: rRNA reduction with double stranded nuclease

Chung et al. (2015)

7: PCR Amplification

8: Next generation sequencing

References

Chung, Betty Y, Thomas J Hardcastle, Joshua D Jones, Nerea Irigoyen, Andrew E Firth, David C Baulcombe, and Ian Brierley. 2015. “The Use of Duplex-Specific Nuclease in Ribosome Profiling and a User-Friendly Software Package for Ribo-Seq Data Analysis.” RNA 21 (10): 1731–45.